구강 편평상피세포암 동위종양 모델에서 전이관련 인자의 발현
Expressions of metastasis-related factors in orthotopic tumor models of oral squamous cell carcinoma
박영욱, 이종원, 김소희,
소속 상세정보
박영욱 ( Park Young-Wook ) - 강릉대학교 치과대학 구강악안면외과학교실
이종원 ( Lee Jong-Won ) - 강릉대학교 치과대학 구강악안면외과학교실
김소희 ( Kim So-Hee ) - 강릉대학교 치과대학 약리학교실
KMID : 0360120080300060529
Abstract
Background and Purpose: Oral squamous cell carcinoma (OSCC) is one of the most aggressive tumors of the head and neck area. OSCC is known to preferentially metastasize via lymphatic system, and resulting cervical lymph node metastasis is the most reliable of treatment failure. But the biological mechanism of the regional nodal metastasis is not clear. So, we determined metastasis-related factors in orthotopic nude mouse models of OSCC.
Experimental Design: Two cell lines-KB and YD-10B cells, established from human oral mucosal squamous cell carcinoma, were xenografted into the tissue space of athymic murine mouth floor. The mice were followed for tumor development and growth, the murine tumors were examined histopathologically for local invasion or regional or distant metastasis. Finally, we performed immunohistochemical assays with antiepithelial growth factor (EGF), EGF receptor (EGFR), phosphorylated EGFR (pEGFR), and anti-vascular endothelial growth factor (VEGF), VEGF receptor (VEGFR)-2, phosphorylated VEGFR-2/3 (pVEGFR-2/3) antibodies. We also determined the microvessel density.
Results: Transplantation of human OSCC tumor cells into the mouth floor successfully resulted in the formation of orthotopic tumors. KB cell line showed significantly higher tumor proliferation and higher nodal metastatic potential than YD-10B cell line. Furthermore, immunohistochemical staining demonstrated higher expression of EGFR/pEGFR, VEGF, and pVEGFR-2/3 as well as higher microvessel density in KB murine tumors than in YD-10B murine tumors.
Conclusion: An orthotopic model of OSCC in athymic mice was established which copies the cervical lymph nodal metastasis of human OSCC. Our mouth floor model should facillitate the understanding of the molecular pathogenesis of cervical nodal metastasis of OSCC.
키워드
Oral squamous cell carcinoma;Lymph node metastasis;Metastasis-related factors;Mouth floor model
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